Effects of estrogen on osteogenesis and fibroblast-related gene expression of bone marrow mesenchymal stem cells in vitro

doi:10.3969/j.issn.2095-4344.2016.33.001

Abstract

Abstract:

BACKGROUND: Bone marrow mesenchymal stem cells have been shown to be differentiated into periodontal ligament fibroblasts when co-cultured with periodontal ligament cells. Existing studies have shown that estrogen has the ability to influence bone marrow regeneration.
OBJECTIVE: To investigate the effects of estrogen on osteogenesis and fibroblast-related factors alkaline phosphatase, type I and III collagen in bone marrow mesenchymal stem cells.
METHODS: Bone marrow mesenchymal stem cells isolated from Beagle dogs were treated with estrogen. Osteogenesis and fibroblast-related mRNA and protein expression of bone marrow mesenchymal stem cells was determined by RT-PCR and western blot assay, respectively.
RESULTS AND CONCLUSION: mRNA and protein expression of type I and III collagen in bone marrow mesenchymal stem cells was upregulated following estrogen treatment; especially, in contrast with type III collagen, the changes of type I collagen were more obvious. Estrogen did not influence mRNA and protein expression of alkaline phosphatase. These findings suggest that estrogen promotes the differentiation of bone marrow mesenchymal stem cells into fibroblasts, whereas does not impact the genes involved in parodontium mineralization.

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

Key words: Estrogens, Mesenchymal Stem Cells, Alkaline Phosphatase

CLC Number:

R318

Peng Wei, Qin Yuan, Li Kun-he, Chen Song-ling. Effects of estrogen on osteogenesis and fibroblast-related gene expression of bone marrow mesenchymal stem cells in vitro[J]. Chinese Journal of Tissue Engineering Research, 2016, 20(33): 4869-4875.

Figures/Tables 2

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